Coding

Part:BBa_K4772001:Design

Designed by: 清雯 李   Group: iGEM23_Tsinghua-TFL   (2023-10-08)

ChlF: A photo-oxidoreductase that uses light to oxidize chlorophyll a to produce chlorophyll f.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 913
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part's sequence do not contain illegal restriction sites (or other specified sequences) as defined by the assembly standard. And it is compatible to iGEM Type II S Assembly Method.

Source

The sequence of ChlF is from Chlorogloeopsis fritschii PCC 9212 .The specific website address is as follows: https://www.ncbi.nlm.nih.gov/protein/1099384185. After we get the sequence, it underwent codon optimization according to Chlamydomonas reinhardtii and was synthesized by company.

References